Thread by @BidoliNicola, Identification of the VIRAL ENVELOPE in SC21) If we analyze the CD21480 [...]

Identification of the

VIRAL ENVELOPE in SC2

1) If we analyze the CD21480 file, of SARS CoV 2 Spike S1-RBD, we can understand how much the binding domain of the receptor of the S1 subunit, of the Spike (S) protein, contains a list of highly

2) pathogenic infectious agents.

CoV S protein is an envelope glycoprotein that plays the most important role in viral attachment, fusion and entry into host cells and serves as a primary target for the development of neutralizing antibodies, viral entry inhibitors

3) and vaccines.

It is synthesized as a precursor protein that is cleaved into an N-terminal S1 subunit (700 amino acids) and a C-terminal S2 subunit (600 amino acids) which mediates membrane attachment and fusion, respectively.

4)

Three S1 / S2 heterodimers assemble to form a trimer peak protruding from the viral envelope.

The S1 subunit contains a receptor binding domain (RBD), while the S2 subunit contains a hydrophobic fusion peptide and two heptad repeat regions.

5)

S1 contains two structurally independent domains, the N-terminal domain (NTD) and the C-terminal domain (domain C).

Depending on the virus, the NTD or the C domain can act as a receptor binding domain (RBD).

6)

While murine hepatitis virus (MHV) RBD is found in the NTD, most other CoVs, including SARS-CoV-2, use the C domain to bind their own receptors.

Recent studies have found that the receptor-binding domain (RBD) of the SC2-S protein binds strongly to human and bat

7) angiotensin-converting enzyme 2 (ACE2) receptors.

Furthermore, SARS-CoV-2 RBD showed significantly higher binding affinity for the ACE2 receptor than SARS-CoV RBD.

Due to the key role of the RBD protein S in viral attachment, it is the main target for

8) antibody-mediated neutralization.

Below, in this link, you can read the sequence alignment of SARS CoV 2 Spike S1-RBD:

https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=394827

Continuing, through a comparative research, a document by Shi Z. emerged. = ( https://www.nature.com/articles/s41586-020-2012-7 )

A pneumonia outbreak associated with a new coronavirus of probable bat origin

Characterization of full-length genome sequences from patients infected with a new coronavirus (2019-nCoV) shows that the sequences are nearly identical and indicates that the virus is related to a...

https://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=394827

9) in which the complete genomic sequence of the

WIV02 virus obtained from a patient is provided hit on

December 30, 2019 and hospitalized at

Whuan Jihyintan hospital.

The paper refers to the Gen Bank Archive,spike glycoprotein Severe acute respiratory

10) syndrome coronavirus 2:

https://www.ncbi.nlm.nih.gov/protein/1807246650

In the paper by Shi Z., under the item Availability of data, you can read:

Sequence data supporting the results of this study were filed in GISAID ( https://www.gisaid.org/ ) with membership numbers

11) with membership numbers EPI_ISL_402124,
EPI_ISL_402127 - EPI_ISL_402130, and EPI_ISL_402131;

GenBank with access numbers MN996527 -
MN996532;

National Genomics Data Center, Beijing Institute of Genomics, Chinese Academy of Sciences

( https://bigd.big.ac.cn/databases?lang=en)

12) with access numbers: SAMC133236 -
SAMC133240, and
SAMC133252.

(Insights: Shi Z. has carried out several projects, with

WIV02, from which new subsequent experiments with new WIV viruses are generated:

https://bigd.big.ac.cn/biosample/browse/SAMC133236

https://bigd.big.ac.cn/gsa/browse/CRA002423

13)

https://bigd.big.ac.cn/gsa/browse/CRA002423/CRX097477 )

Let's take into consideration the access number to Gen Bank:

MN996527 (MN996532 instead belongs to the RaTG13 virus which is actually a "visual diversion", created to divert attention from the WIV virus family), in which you can read its

14) entire genomic sequence:

https://www.ncbi.nlm.nih.gov/nuccore/MN996527

The virus WIV02: Severe acute respiratory syndrome coronavirus 2 isolate WIV02, complete genome, then refers to the following link:

https://webcache.googleusercontent.com/search?q=cache:B84T821B7F8J:https://bigd.big.ac.cn/gwh/Assembly/952/show+&cd=1&hl=it&ct=clnk&gl=it

15)

16) This intermediate link is very important because it provides a first cognitive approach to who created it and how it was developed:

Scientific name Severe acute respiratory syndrome coronavirus 2

Common name Bioproject PRJCA002163

Biosample SAMC133236

17)

Membership n. GWHABKK00000000

Raw GSA readings - Variations GVM16.114 (SNP),820 (Indel)

Sender Organization

CAS Key Laboratory of Special Pathogens and Biosafety and Center for Emerging Infectious Diseases

Wuhan Institute of Virology,Chinese Academy of Sciences

18)

Contact: Peng Zhou, peng.zhou@wh.iov.cn Sequence authors Peng Zhou, Xinglou Yang, Xianguang Wang, Lei Zhang, Yan Zhu, Haorui Si, Zhengli Shi.

Issued 01-30-2020

Complete genome assembly level

Representation of the genome Complete genome

Reference genome, yes

19)

Geneious assembly method v11.0.3, Megahit v1.2.9

Sequencing and coverage Illumina MiSeq 74862.73271, MGISEQ 2000 6.747680972E9

Finally WIV02 then refers to Wuhan-Hu-1, where you can read, in Gen Bank, the entire sequence:

https://www.ncbi.nlm.nih.gov/nucleotide/MN908947

20)

Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome.

And it is here, in this link, that you can go back, in a direct and precise way, to obtaining the viral envelope of SC2 and its incredible list of highly pathogenic infectious agents:

21)

https://www.viprbrc.org/brc/viprDetails.spg?ncbiProteinId=QHD43416&decorator=corona

I attach the graphic scheme consisting of the entire genomic sequence, of Wuhan-Hu-1, with its double amino acid / protein codon.

22)

Scrolling through the list of various items, you get to the item, Gene Composition, where under the item: Cellular Component you can read:

VIRAL ENVELOPE.

22) Scrolling through the list of various items, you get to the item, Gene Composition, where under the item: Cellular Component you can read: VIRAL ENVELOPE.

23)

24)

25)

Thanks to the code of the viral envelope: 0019031, it was possible to trace the exact composition of the pathogens present in Viral Enveloped (N), of SC2:

https://www.ebi.ac.uk/QuickGO/term/GO:0019031

This link is a “mine” of information, disconcerting and incredible, which I have named

26) "Aladdin's Cave", it is the "Secretum Secretorum" of the chimeric virus SC2 where it is listed, one by one, all the infectious pathogens present in large quantity, both in S1 and in N.

26)

27)

28)

In addition to the already disconcerting viral envelope belonging to N, it was also possible to trace the exact composition of the pathogens present in the trimerium S1, S2, of SC2, thanks to this sub-link:

https://www.ebi.ac.uk/QuickGO/term/GO:0044423

In the viral envelope:

0044423,

29) Virion Component, you can read the viral composition of S1 / Spike:

30)

31)

CONCLUSIONS / CONSIDERATIONS,PATHOGENS COMPONENTS IN THE VIRAL ENVELOPE.

INFLUENCE A/B/C=The co-presence of all three influenza viruses explains how they have been skilfully used,under the radar,by SC2;forming a highly infectious pelvis,especially in frail and elderly

32) people.

FLAVIVIRUS NS4A / NS2B = ssRNA positive single-stranded RNA virus, belonging to the Flaviviridae family. They result in a variety of different diseases with fever, sometimes severe encephalitis and / or hemorrhagic fever. Dengue virus, Zika, and

33) yellow fever also belong to this family. The Flaviivrus virus is divided into 2 clades: the first is given by viruses transmitted by a vector and while the second clade is given by viruses without the known vector. The vector clade, in turn, can be divided into a

34) mosquito-borne clade and a tick-borne clade. The mosquito group can be divided into two branches: one branch contains neurotropic viruses, often associated with encephalitic disease in humans or livestock. The second branch is given by non-neurotropic viruses

35) associated with haemorrhagic disease in humans and primates.

PNEUMOVIRUS = RNA negative single-stranded virus, belonging to the order Mononegavirales, family Pneumoviridae. The type species is the human respiratory syncytial virus, an important causative

36) agent of both infantile bronchiolitis and pneumonia.

ARENAVIRUS = RNA virus, ambisense, which infects rodents and occasionally humans. Arenaviruses cause human disease and vary in severity. Aseptic meningitis, a serious human disease that causes

37) inflammation that covers the brain and spinal cord, can result from the lymphocytic choriomeningitis virus. Hemorrhagic fever syndromes, including Lassa fever, are derived from infections such as Guanarito virus, Junin virus, Lassa virus, Lujo

38) virus, Machupo virus, Sabia virus, or Whitewater Arroyo virus. Glycoprotein (GP) is synthesized as a precursor molecule. It is divided into three parts: GP1, GP2 and a stable signal peptide (SSP). These reactions are catalyzed by cell signal peptidases and the

39) cellular enzyme Subtilisin Kexin Isozyme-1 (SKI-1) / Site-1 in Protease (S1P). These processes are essential for fusion competence and the incorporation of mature GP into nascent virion particles.

PARAMIXOVIRUS = have a single-stranded RNA. Parainfluenza viruses, mumps

40) virus, measles virus and human respiratory syncytial virus belong to this family.

HIV Gp120 = Gp120 is a viral glycoprotein present in the pericapsid of the HIV virus, It is an essential protein for the entry of the virus into cells, it plays a

41) fundamental role in cell adhesion through receptors of the DC-SIGN type, heparan sulfate and a specific interaction with CD4 of helper T lymphocytes.

EIAV Gp90 / Gp2b = equine infectious anemia, classified in the family Retroviridae, in the

42) subfamily Lentivirinae (which also includes the sheep Visna-maedi viruses, arthritis - caprine encephalitis, and the viruses responsible for the infection of humans, monkeys, felines and bovine immunodeficiency) .

HEPATITE C = HCV belongs to the hepacivirus genus in the

43) Flaviviridae family and primarily affects the liver. Co-infection with HIV is very common.

HERPESVIRUS = Herpes simplex virus (Varicella-zoster virus); latency site: nerve endings of the CNS. Characteristic of this family of viruses is that it no

44) longer leaves the host after the first infection and nestles in a cell type of the organism, causing a so-called latent infection. This family is also linked to the Epstein-Barr virus; its seat of latency lies in the lymphoid cells.

CITOMEGALOVIRUS = The

45) genus includes viruses that infect various species of primates, including humans. The two cases in which serious complications can occur are the pregnant patient and the immunocompromised patient. The latter, for example, can undergo chorioretinitis,

46) esophagitis, hepatitis and, especially for reactivation of the virus from the latency phase, encephalitis and pneumonia.

ARTERIVIRUS Cp4 = These proteins, collectively referred to as "replicase / transcriptase" or, for simplicity, simply "replicase", are

47) encoded by open read frames 1a and 1b (ORF1a and ORF1b) of the arterivirus genome. Furthermore, the family includes viruses of veterinary interest with high genomic variability, capable of causing persistent infections with prolonged viremia and the ability to replicate in

48) macrophages. (It is no coincidence that this virus is always found in laboratory experiments carried out with animals in vivo).

CHORDOPOXVIRUS = This is a Chordopoxvirinae family of viral fusion proteins. A Vaccinia virus protein has been shown to

49) function as a viral fusion protein that mediates cell fusion.

PROXVIRUS P35 / Ag35 / A14 / A28 = The smallpox and alastrim viruses also belonged to this family, diseases that have now been eradicated, but still strangely present in the viral envelope of SC2.

50)

BACULOVIRUS Gp64 / E56 / E66 / E25 / EC27 / E18 = The envelope fusion protein GP64 is an essential baculovirus protein required for cell-to-cell infection transmission. Baculoviruses infect insects (such as mosquitoes, linked to malaria), with over 600

51) host species. In the 1940s, baculovirus was widely used and studied as a biopesticide in cultivated fields. Since the 1990s they have been used to produce complex eukaryotic proteins in insect cell cultures (Sf21, High Five cells). These recombinant proteins have been used in

52) research and as vaccines in both human and veterinary medical treatments (e.g., the most widely used vaccine for the prevention of H5N1, avian influenza in chickens that has been produced in a baculovirus expression vector ). More recently it has been discovered that

53) baculoviruses can also be transduced into mammalian cells, through the use of VLP / Sf21 particles suspended in a yeast culture inside which mammalian cells are present.

CORE Gp120 = Gp120 is a viral glycoprotein present in the pericapsid of the HIV virus. It is an

54) essential protein for the entry of the virus into cells, it plays a fundamental role in cell adhesion via DC-SIGN type receptors, heparan sulfate and a specific interaction with CD4 of helper T lymphocytes.

RHABDOVIRUS = Bats are carriers of the Lyssavirus, or

55) rabies virus, which is responsible for rabies and vesicular stomatitis in cattle and horses. The viral particle is wrapped in a fatty membrane; it is shaped like a “bullet” (70 by 180 nm) and contains a single helical strand of ribonucleic acid (RNA).

56) The pathogen of rabies is a Lyssavirus with different genotypes; Genotype 1 is the classic rabies virus that can also cause vesicular stomatitis.

VSV MATRIX = The vesiculovirus matrix protein family consists of several matrix proteins of the vesicular

57) stomatitis virus, also known as VSIV or VSV. The matrix (M) protein of the virus causes many of the cytopathic effects of VSV, the M protein also induces apoptosis (cell death). These proteins play an important role in the assembly and budding of VSIV

58) virions. Their main role is to help assemble the virus. They begin by disrupting host cell transcription by inhibiting nuclear export of mRNA through direct interaction with the host's RAE1 -NUP98 complex. This inhibits the signaling of interferon and thus

59) the creation of an antiviral state in virus-infected cells. In turn, this induces cell rounding, disorganization of the cytoskeleton and apoptosis in the infected cell.

VESICULOVIRUS = As with RHABDOVIRUS, this virus is linked to the rabies virus, it also causes

60) vesicular stomatitis.

TROMBUSVIRUS P19 = RNA silencing suppressor p19 (also known as the Tombusvirus P19 core protein and 19 kDa symptom severity modulator) is a protein expressed by the ORF4 gene in the tombusvirus genome. The p19 protein acts as a counter-defense

61) strategy, specifically binding 19 to 21 nucleotide double-stranded RNAs that function as small interfering RNAs (siRNAs) in the RNA silencing system. By sequestrating siRNA, p19 suppresses RNA silencing and promotes viral proliferation. By binding to siRNA, p19

62) sequestrates these species and prevents them from interacting with the RNA-induced silencing complex (RISC), a protein complex that mediates the antiviral RNA silencing mechanism in the cell.

63)

FINAL:

All these pathogens, now well identified in the Viral Envelope of SC2, have similar and almost always recurring correspondences, which unite them all, as if they belonged to a cleverly studied viral project, with a very specific purpose.

64) The pharmacological response in medical protocols, in response to SC2, should be very high but, on the contrary, there have been deficiencies in the use of unsuitable drugs, increasing lethality in the population.

For example, it is no coincidence

that the understanding of the mechanism of action of bacterial lysates (specialties of medicines that contain bacterial antigens, i.e. fragments of bacteria, or polypeptide chains which, if taken by a healthy organism, should stimulate its immune response )

is the main cause of relapse in severe respiratory infections, such as acute pneumonia, and therefore its use should be prohibited.

In fact, inside the lysates there are endogenous microsomes with an active translocon complex (such as vesicular cellular artifacts

derived from the fragmentation of the endoplasmic reticulum in the laboratory) which facilitate the incorporation of the bacterial channel of potassium KcsA (ion channels that allow the passage of cations of potassium through the plasma membrane of the

cells), within the microsomal membranes themselves.

This would explain very well how, after the appearance of SC2, there was an exponential increase in the number of bacterial strains resistant to antibiotics, especially Gram negative.

SC2, due to its zoonotic / chimeric origin and its "parasitic nature", in addition to creating a very serious syndrome, which attacks both the vascular system and the immune system, binds and spreads mainly through bacterial, intestinal and external colonies to the guest.

For this reason, precisely due to the presence of the Viral Envelope present in SC2, all immunological control strategies of infectious diseases should be re-evaluated instantly.
(End)

@threadreaderapp
Unroll

Latest Threads Unrolled: