I am posting a list of most of the female video game characters I have played in my life. Thread will be updated at least once a day and will start off in alphabetical order. No custom characters a la FemShep, though I love her. Other arbitrary rules in effect too. Let's begin.

Female Video Game Characters I have played as #1:

2B - Nier: Automata
A2 - Nier: Automata
Abby - The Last of Us Part 2
Ada Wong - Resident Evil 2

Female Video Game Characters I have played as #2:

Alex - Oxenfree
Alexandra Roivas - Eternal Darkness: Sanity's Requiem
Aloy - Horizon Zero Dawn
Alyson Ronan - Tell Me Why

Female Video Game Characters I have played as #3:

Amanda Ripley - Alien: Isolation
Amicia de Rune - A Plague Tale: Innocence
Amitjyoti "Amy" Ferrier - Tacoma
April Ryan - The Longest Journey

Female Video Game Characters I have played as #4:

Ashley - Until Dawn
Athena - Borderlands: The Pre-Sequel
Aurelia Hammerlock - Borderlands: The Pre-Sequel
Aya Brea - Parasite Eve

Female Video Game Characters I have played as #5:

Billie Lurk - Dishonored: Death of the Outsider
Carla Valenti - Indigo Prophecy
Cassie - Uncharted 4: A Thief's End
Cate Archer - The Operative: No One Lives Forever

Female Video Game Characters I have played as #6:

Catwoman - Batman: Arkham City
Chell - Portal
Chloe Price - Life is Strange: Before the Storm
Ciri - The Witcher 3: Wild Hunt

Female Video Game Characters I have played as #7:

Claire - A Short Hike
Claire Redfield - Resident Evil 2
Clementine - The Walking Dead
Dixie Kong - Donkey Kong Country 2: Diddy's Kong Quest

Female Video Game Characters I have played as #8:

Edith Finch - What Remains of Edith Finch
Elizabeth - BioShock Infinite: Burial at Sea
Ellie - The Last of Us
Elodie - Long Live the Queen

Female Video Game Characters I have played as #9:

Emily Davis - Until Dawn
Emily Kaldwin - Dishonored 2
Eva Rosalene - To the Moon
Faith Connors - Mirror's Edge

Female Video Game Characters I have played as #10:

Fiona - Tales from the Borderlands
Gaige - Borderlands 2
Gemma - Ninja Pizza Girl
Gris - Gris

Female Video Game Characters I have played as #11:

Jade - Beyond Good & Evil
Jesse - Minecraft: Story Mode
Jesse Faden - Control
Jessica Harding - Jurassic Park: The Game

Female Video Game Characters I have played as #12:

Jessica Riley - Until Dawn
Jill Valentine - Resident Evil
Jodie Holmes - Beyond: Two Souls
Julianne Stingray - VA-11 Hall-A

Female Video Game Characters I have played as #13:

Juliet Starling - Lollipop Chainsaw
Kaede Akamatsu - Danganronpa V3
Kaitlin Greenbriar - Gone Home
Kara - Detroit: Become Human

Female Video Game Characters I have played as #14:

Kate Diaz - Gears 5
Kay - Sea of Solitude
Kim Pine - Scott Pilgrim vs the World: The Game
Lara Croft - Tomb Raider

Female Video Game Characters I have played as #15:

Lili - Lili: Child of Geos
Lilith - Borderlands
Liz Allaire - The Next Big Thing
Madeline - Celeste

Female Video Game Characters I have played as #16:

Madison Paige - Heavy Rain
Mairu Hibisu - Cherry Tree High Comedy Club
Marianne Rekowicz - The Medium
Marina Liteyears - Mischief Makers

Female Video Game Characters I have played as #17:

Max Caulfield - Life is Strange
Maya - Borderlands 2
Michonne Hawthorne - The Walking Dead: Michonne
Miku - Submerged

Female Video Game Characters I have played as #18:

Mira Forrester - Game of Thrones
Moze - Borderlands 3
Nilin Cartier-Wells - Remember Me
Nima Cruz - Jurassic Park: The Game

Female Video Game Characters I have played as #19:

Peach - Super Mario Bros. 2
Razor - Maniac Mansion
Regina - Dino Crisis
Samantha Giddings - Until Dawn

Female Video Game Characters I have played as #20:

Samus Aran - Metroid
Scout - Flame in the Flood
Senua - Hellblade: Senua's Sacrifice
Shannon Marquez - Kentucky Route Zero

Female Video Game Characters I have played as #21:

Sherry Birkin - Resident Evil 2
Tiny Kong - Donkey Kong 64
Weiss Schnee - RWBY Grimm Eclipse
Wendy - Maniac Mansion

Female Video Game Characters I have played as #22:

Wren - Costume Quest
Zoe Castillo - Dreamfall: The Longest Journey
Joanna Dark - Perfect Dark
Korra - The Legend of Korra

I will continue this thread by adding any characters I missed on my first pass, like Joanna Dark and Korra, plus including RPG party members that can be controlled in battle.