Do you know anyone who has been tested #COVID19 positive from one lab & negative from another? In Pakistan, one of the many reasons for such discrepancies is the confusion over the Ct values –metric based on which our COVID positivity status is determined. A 🧵on Ct values
#CtValue refers to the number of cycles needed to amplify viral RNA to reach a detectable level. Ct value is inversely proportional to the patient’s viral load. I made this video clip explaining Ct values.
There are different kits available for the COVID19 RT-PCR test. Most tests use a 35-cycle protocol. If the virus isn’t detected within 35-amplification cycles, the test result -ve. If it is detected, then the test is +ve.
The scientists are debating over the Ct values. A few have claimed that 35 is too high, and patients are not likely to be infectious at such higher Ct values.
But there are different scenarios where a high Ct value may feature. For, e.g., Pre-symptomatic infection that subsequently develops into symptomatic infection with high viral load and infectivity.
https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/926410/Understanding_Cycle_Threshold__Ct__in_SARS-CoV-2_RT-PCR_.pdf
The problem is that several diagnostic labs give a negative result when the Ct value is higher, and the patient is asymptomatic. Especially to the ones that are getting it done for traveling.
One of my colleagues spoke to a few health department officials –responsible for keeping an eye on the diagnostic facilities– about Ct values. Much to our dismay, they were not even aware of what Ct values are.
🤯
And if we give negative results to potentially infectious patients, the virus will spread. I understand that due to the lack of standardization across tests, most labs shy away from reporting the Ct values. And the test results are only viewed in a binary way — positive/negative.
We @CancerbioC @pu_lhr_official are working on CT values & their association with disease severity in Pakistani patients. We will showcase our work in the coming days. For possible collaborations, please DM or send an email to director.crc@pu.edu.pk. @WHOPakistan @NIH_Pakistan
You can follow @Nosheen_Z.
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