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One thing apparent when re-visiting the history of plant microscopy, is that microscopists have always loved the fine structure of the cytoskeleton. Another little thread for this favorite of microscopists. 
https://twitter.com/somssichm/status/1386558825088552962
https://twitter.com/somssichm/status/1386558825088552962
In 1977 Klaus Weber started to raise antibodies against several cytoskeleton proteins. The textbook view of the cytoskeleton was based on his images for decades. In 1977, plant microscopists used his anti-tubulin to get a first glance of the plant cytoskeleton. [1] (Refs at end)
![In 1977 Klaus Weber started to raise antibodies against several cytoskeleton proteins. The textbook view of the cytoskeleton was based on his images for decades. In 1977, plant microscopists used his anti-tubulin to get a first glance of the plant cytoskeleton. [1] (Refs at end)](https://pbs.twimg.com/media/Ez4ZwtgVkAAcM0k.jpg "In 1977 Klaus Weber started to raise antibodies against several cytoskeleton proteins. The textbook view of the cytoskeleton was based on his images for decades. In 1977, plant microscopists used his anti-tubulin to get a first glance of the plant cytoskeleton. [1] (Refs at end)")
The newly invented confocal microscope was used in 1990 to image microinjected fluorescently-labeled pig tubulin in the plant's microtubule network during cell division. [2]
![The newly invented confocal microscope was used in 1990 to image microinjected fluorescently-labeled pig tubulin in the plant's microtubule network during cell division. [2]](https://pbs.twimg.com/media/Ez4aWI0UUAA1E1s.jpg "The newly invented confocal microscope was used in 1990 to image microinjected fluorescently-labeled pig tubulin in the plant's microtubule network during cell division. [2]")
Then, in 1993, the incorporation of the microinjected tubulin into the microtubule-network was live-imaged, as well as the dissolution and recovery of the network following treatment with the herbicide oryzalin. [3]
![Then, in 1993, the incorporation of the microinjected tubulin into the microtubule-network was live-imaged, as well as the dissolution and recovery of the network following treatment with the herbicide oryzalin. [3]](https://pbs.twimg.com/media/Ez4afOzVgAc0_xH.jpg "Then, in 1993, the incorporation of the microinjected tubulin into the microtubule-network was live-imaged, as well as the dissolution and recovery of the network following treatment with the herbicide oryzalin. [3]")
When the plant-compatible GFP came along in 1996/7, the microtubules were of course among the first structures to be imaged, this time using the Microtubule Binding Domain (MBD)-protein. [4]
![When the plant-compatible GFP came along in 1996/7, the microtubules were of course among the first structures to be imaged, this time using the Microtubule Binding Domain (MBD)-protein. [4]](https://pbs.twimg.com/media/Ez4bL1iVoAMM6Bm.jpg "When the plant-compatible GFP came along in 1996/7, the microtubules were of course among the first structures to be imaged, this time using the Microtubule Binding Domain (MBD)-protein. [4]")
And obviously, the cytoskeleton was also a favorite when the new super-resolution techniques had to be establied for plant cells, for example to image tubulin via SIM... [5]
![And obviously, the cytoskeleton was also a favorite when the new super-resolution techniques had to be establied for plant cells, for example to image tubulin via SIM... [5]](https://pbs.twimg.com/media/Ez4b-mgUcAEvr2u.jpg "And obviously, the cytoskeleton was also a favorite when the new super-resolution techniques had to be establied for plant cells, for example to image tubulin via SIM... [5]")
...or the AiryScan for the same. [6]
![...or the AiryScan for the same. [6]](https://pbs.twimg.com/media/Ez4cTvqUYAIr7Ox.jpg "...or the AiryScan for the same. [6]")
But even a spinning-disc with a super-fast camera can capture close to super-resolution images... and videos. Shown here, the rearrangement of individual microtubules into thick, regularly spaced bundles for secondary cell wall pattern formation in single cells in planta. [7]
REFs: [1] W. W. Franke et al. (1977) Cell Biology International Reports [2] https://doi.org/10.1073/pnas.87.22.8820 [3] https://doi.org/10.1002/cm.970240308 [4] https://doi.org/10.1105/tpc.10.11.1927 [5] https://doi.org/10.3389/fpls.2017.00866 [6] https://doi.org/10.3389/fpls.2020.00693 [7] https://www.nature.com/articles/s41467-021-20894-1
Since some of the people who produced the images/video in this thread are actually on Twitter, I would like to add that [3] was done by @GWasteneys et al., [5&6] are by @Samaj_lab et al., and the video in [7] is from @Schneider_Lab_ et al. So if you like the images, follow them.
