1/ TEN SCIENTIFIC REASONS IN SUPPORT OF AIRBORNE TRANSMISSION OF SARS-CoV-2

Peer-reviewed publication in @TheLancet

An honor to have collaborated in multidisciplinary team across medicine, infectious diseases, epidemiology, aerosol science, sociology https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(21)00869-2/fulltext">https://www.thelancet.com/journals/...
Ten scientific reasons in support of airborne transmission of SARS-CoV-2

Heneghan and colleagues' systematic review, funded by WHO, published in March, 2021, as a preprint, states: “The lack of recoverable viral culture samples of SARS-CoV-2 prevents firm conclusions to...

https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(21)00869-2/fulltext
2/ The authors are:

- @trishgreenhalgh, Prof. of Medicine at Oxford Univ., pioneer of evidence-based medicine (EBM)
- @DFisman, Prof. at Univ. of Toronto, epidemiologist
- @chipatucsd, Prof. of Medicine at UC-San Diego, Chief Editor of journal "Clinical Infectious Diseases"
3/ The rest of the authors:

- @kprather, Prof. UC-San Diego, member of US National Academies of Science and of Engineering, aerosol scientist
- @zeynep, Prof. Univ. of North Carolina, sociologist
- Yours truly, Prof. Univ. of Colorado, Highly Cited Scientist, aerosol scientist
4/ We review ten different lines of evidence that support that SARS-CoV-2 is predominately airborne

I will review the the lines in this thread, with links to key references, and some additional detail to make it more understandable.
5/ FIRST: lots of superspreading events, & the ones that have been studied in detail are best explained by airborne transmission

To our knowledge NOT a single peer-reviewed pub. of an outbreak reports w/ certainty likely droplet or surface transmission being major
6/ An example is the Skagit Choir case that we investigated (led by @ShellyMBoulder, paper here, read the Supp. Info. for the real details: https://doi.org/10.1111/ina.12751),">https://doi.org/10.1111/i... that I summarized in this thread: https://twitter.com/jljcolorado/status/1306450428867964930">https://twitter.com/jljcolora...
7/ Some people argue that superspreading events are interesting but minor overall. That is not correct. Superspreading plays a major part on pandemic spread, and controlling SSE is key to control the pandemic, as @nature summarizes here: https://www.nature.com/articles/d41586-021-00460-x">https://www.nature.com/articles/...
Superspreading drives the COVID pandemic — and could help to tame it

Uneven transmission of the SARS-CoV-2 coronavirus has had tragic consequences — but also offers clues for how best to target control measures.

https://www.nature.com/articles/d41586-021-00460-x
8/ SECOND LINE OF EVIDENCE: growing number of cases of long distance transmission. e.g. in quarantine hotels in Australia and New Zealand: https://wwwnc.cdc.gov/eid/article/27/5/21-0514_article">https://wwwnc.cdc.gov/eid/artic...
Transmission of Severe Acute Respiratory Syndrome Coronavirus 2 during Border Quarantine and Air...

Transmission of SARS-CoV-2 during Air Travel

https://wwwnc.cdc.gov/eid/article/27/5/21-0514_article
9/ Another recently-published case in which an infected person transmitted to several people who were 15 meters BEHIND. Genome sequencing shows that it was the same virus. Video shows that there was no close contact.

https://wwwnc.cdc.gov/eid/article/27/6/21-0465_article">https://wwwnc.cdc.gov/eid/artic...
10/ Why are most of the long-distance transmission cases being identified in the quarantine hotels in Australia & New Zealand?

Because the lack of community cases makes it much more certain that transmission happened there. (Can& #39;t do in US)

Plus there is video surveillance etc
11/ Similarly, airborne transmission of smallpox was debated for centuries, but could only be definitely proven in the complete absence of community transmission

Pakistani electrician arrived in Germany, where there were no cases. Obvious long distance T

https://academic.oup.com/aje/article/93/4/234/230121">https://academic.oup.com/aje/artic...
12/ THIRD LINE OF EVIDENCE: people w/o symptoms transmit 30-59% of the cases.

They don& #39;t cough or sneeze, so they produce very few large droplets. But they go around with their lives and produce lots of aerosols speaking etc. https://jamanetwork.com/journals/jamanetworkopen/fullarticle/2774707">https://jamanetwork.com/journals/...
SARS-CoV-2 Transmission From People Without COVID-19 Symptoms

This decision analytical model assesses the proportion of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmissions in the community that likely occur from persons without symptoms.

https://jamanetwork.com/journals/jamanetworkopen/fullarticle/2774707
13/ FOURTH LINE OF EV.: transmission is 20 times more efficient indoors than outdoors.

Same gravity indoors and outdoors, large droplets do the same in either (can& #39;t explain difference)

Huge aerosol dilution outdoors vs. trapping indoors can explain it https://academic.oup.com/jid/article/223/4/550/6009483">https://academic.oup.com/jid/artic...
Outdoor Transmission of SARS-CoV-2 and Other Respiratory Viruses: A Systematic Review

This systematic review found that while outdoor environments do seem at lower risk for transmission of SARS-CoV-2 and other respiratory viruses than indoor envi

https://academic.oup.com/jid/article/223/4/550/6009483
14/ FOURTH AND A HALF: ventilation of indoor spaces reduces transmission significantly. This is accepted by e.g. @WHO, which recently published a Roadmap to reduce transmission w/ ventilation.

Ventilation impacts airborne, NOT droplets or surfaces https://www.who.int/publications/i/item/9789240021280">https://www.who.int/publicati...
Roadmap to improve and ensure good indoor ventilation in the context of COVID-19

This roadmap aims to define the key questions users should consider to assess indoor ventilation and the major steps needed to reach recommended ventilation levels or simply improve indoor air...

https://www.who.int/publications/i/item/9789240021280
15/ I have yet to hear an attempt at explaining why (f large droplets dominate transmission as @WHO and @CDCgov say) there is such a large indoor / outdoor difference in transmission, or such an impact of ventilation.

Strongly implies airborne is PREDOMINANT mode of transmission
16/ FIFTH: studies have demonstrated (e.g. at Harvard Univ. hospital -- BWH) that health care workers were infected despite contact and droplet protections and eye protection. Genomic match

Surgical masks protect well, but not perfectly against aerosols

https://www.acpjournals.org/doi/full/10.7326/M20-7567">https://www.acpjournals.org/doi/full/...
17/ SIXTH: viable SARS-CoV-2 virus has been detected in the air from two hospitals and a car where infected person was present.

And this has NEVER been achieved for measles or tuberculosis, two accepted airborne diseases.

https://www.ijidonline.com/article/S1201-9712(20)30739-6/fulltext">https://www.ijidonline.com/article/S...
18/ SEVENTH LINE OF EVIDENCE: virus material has been detected via PCR on HVAC ducts and filters of a hospital. It could only have gotten there if it was floating in the air as an aerosol.

https://www.nature.com/articles/s41598-020-76442-2">https://www.nature.com/articles/...
19/ EIGHTH LINE OF EVIDENCE: airborne transmission has been demonstrated with animal models such as ferrets and hamsters.

https://www.nature.com/articles/s41467-021-21918-6">https://www.nature.com/articles/...
20/ Of note is that demonstration of airborne transmission with an animal model (guinea pigs) by Richard Riley and Williams Wells in 1961 is what led to the acceptance that tuberculosis was transmitted through the air, after 5 decades of denial.

https://www.atsjournals.org/doi/10.1164/arrd.1962.85.4.511">https://www.atsjournals.org/doi/10.11...
21/ NINTH: no published studies with strong evidence that contradicts airborne transmission.

There are cases of shared air w/o transmission, but they are easily explained by very large variation in virus emission among people & in time, + ventilation

https://ehp.niehs.nih.gov/doi/10.1289/EHP7886">https://ehp.niehs.nih.gov/doi/10.12...
22/ Some people argue that R0 would be higher if SARS-CoV-2 was airborne

But tuberculosis is only airborne, and is less contagious than COVID

And R0 is very skewed, many don& #39;t transmit, a few transmit a lot (consistent w/ aerosols w/ variable emission): https://wellcomeopenresearch.org/articles/5-67 ">https://wellcomeopenresearch.org/articles/...
23/ We dispel the myth about R0, as well as other commonly-held myths about airborne transmission in this article in the Journal of Hospital Infection, led by prominent virologist Julian Tang:
https://www.sciencedirect.com/science/article/pii/S0195670121000074?via%3Dihub">https://www.sciencedirect.com/science/a...
24/ TENTH: there is limited evidence to support that another route of transmission is dominant (or I add, even important).

Surfaces: @CDCgov and multiple literature studies have been clear that surface transmission of SARS-CoV-2 is rare: https://www.cdc.gov/coronavirus/2019-ncov/more/science-and-research/surface-transmission.html">https://www.cdc.gov/coronavir...
Coronavirus Disease 2019 (COVID-19)

CDC provides credible COVID-19 health information to the U.S.

https://www.cdc.gov/coronavirus/2019-ncov/more/science-and-research/surface-transmission.html
25/ Here the Editors of @nature were practically yelling (on 2-Feb) to @WHO and @CDCgov to say clearly that surfaces transmit little, and that surface disinfection is not important, and efforts should focus on the air. https://www.nature.com/articles/d41586-021-00277-8">https://www.nature.com/articles/...
Coronavirus is in the air — there’s too much focus on surfaces

Catching the coronavirus from surfaces is rare. The World Health Organization and national public-health agencies need to clarify their advice.

https://www.nature.com/articles/d41586-021-00277-8
26/ There is also no clear evidence of large droplet transmission.

Ease of transmission in close proximity has been attributed (erroneously, see next) since 1910 to large droplets, which fall to the ground in 1-2 m

@WHO video illustrates it perfectly: https://twitter.com/WHO/status/1244258441880797184">https://twitter.com/WHO/statu...
27/ But this is an error! Modern science shows that if a disease is transmitted easily in close proximity while talking, and social distance reduces transmission, that is explained mainly by short-range airborne transmission, and NOT by droplets.

https://www.sciencedirect.com/science/article/pii/S0360132320302183">https://www.sciencedirect.com/science/a...
28/ Ease of transmission in close proximity (along with cases of lack of transmission in shared air, see tweet #21) was used for 7 decades to declare measles a droplet / fomite disease, and to deny its airborne character.

https://pediatrics.aappublications.org/content/75/4/676.long">https://pediatrics.aappublications.org/content/7...
29/ In fact, droplet transmission has NEVER been demonstrated directly for ANY disease in entire history of medicine

(From a review of the literature by Prof. Yuguo Li et al. in this paper: https://www.sciencedirect.com/science/article/pii/S0360132320302183.">https://www.sciencedirect.com/science/a... If you have a paper that proves otherwise, pls send it my way)
30/ So how did ease of infection in close proximity come of be interpreted as PROOF of droplet transmission and evidence against airborne transmission?

Charles Chapin was a prominent American Public Health researcher, 1927 president of American PH Ass.

https://en.wikipedia.org/wiki/Charles_V._Chapin">https://en.wikipedia.org/wiki/Char...
31/ In his 1910 seminar book, "The Sources and Modes of Infection" ( https://archive.org/details/sourcesmodesofin00ch),">https://archive.org/details/s... Chapin misinterpreted the earlier measurements of Flugge in 1890s.

Fluggle waited 5 hrs for aerosols to settle on plates. But got distorted into "sprayborne droplets" by Chapin.
32/ Chapin through contact infection was most important. But observed:

"it is impossible, as I know from experience, to teach people to avoid contact infection while they are firmly convinced that the air is the chief vehicle of infection"

Airborne trans. was getting in his way
33/ He admits that there is no clear evidence against airborne transmission, as aerosol measurements were not developed yet.

Still proceeds to "discard airborne infection.":

"It will be a great relief to most persons to be freed from the specter of infected air"
34/ Unfortunately Chapin is too successful. And even though there was not evidence to show that close contact transmission was explained by Chapin& #39;s "sprayborne droplets", this became a tradition and a dogma.

#DropletDogma
35/ Airborne trans. denied for decades. Wells ( https://en.wikipedia.org/wiki/William_F._Wells),">https://en.wikipedia.org/wiki/Will... Riley fight back.

First director of CDC (A. Langmuir) makes fun of Wells in 1950 for thinking that ANY natural disease can be airborne

We have just submitted a paper on this history, preprint out next wk
William F. Wells - Wikipedia

https://en.wikipedia.org/wiki/William_F._Wells
36/ Eventually the evidence becomes undeniable for a few diseases: TB in 1961 (short story: https://atsjournals.org/doi/full/10.1164/ajrccm.163.1.hh11-00),">https://atsjournals.org/doi/full/... and later for measles and chickenpox.

Note that in 1985 still most public health authorities described measles as droplet/fomite ( https://pediatrics.aappublications.org/content/75/4/676.long)">https://pediatrics.aappublications.org/content/7...
Measles Outbreak in a Pediatric Practice: Airborne Transmission in an Office Setting

In February 1981, a measles outbreak occurred in a pediatric practice in DeKalb County, GA. The source case, a 12-year-old boy vaccinated against measles at 111/2 months of age, was in the office for...

https://atsjournals.org/doi/full/10.1164/ajrccm.163.1.hh11-00
37/ Incidentally, measles and chickenpox were accepted as airborne with A LOT less evidence than we have now for COVID-19.

(From: https://twitter.com/JenniferKShea/status/1373840694176010240)">https://twitter.com/JenniferK...
38/ Airborne transmission is considered very very difficult, little attention devoted to it.

So huge errors (the size of Godzilla) creep into the description of droplets and aerosols in medical textbooks, such as 5 um being the separation (it is 100) https://twitter.com/jljcolorado/status/1295518786951319552">https://twitter.com/jljcolora...
39/ We already knew in 1934 (from Wells& #39; work at Harvard https://academic.oup.com/aje/article-abstract/20/3/611/280025)">https://academic.oup.com/aje/artic... that droplets vs. aerosols boundary was at 100 microns.

Reaffirmed by @theNasem and @ScienceMagazine: https://science.sciencemag.org/content/370/6514/303.2">https://science.sciencemag.org/content/3...
Airborne transmission of SARS-CoV-2

There is overwhelming evidence that inhalation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) represents a major transmission route for coronavirus disease 2019 (COVID-19). There is...

https://academic.oup.com/aje/article-abstract/20/3/611/280025
40/ Back to @TheLancet: "There is consistent, strong evidence that SARS-CoV-2 spreads by airborne trans. Although other routes can contribute, we believe that the airborne route is likely to b dominant. The public health community should act accordingly and without further delay"
41/ If I missed something, if you think there is an error, if you think there is evidence we are not considering, send it my way.

If you know of a paper that reviews as much evidence and explains why it is really droplets, definitely send it my way (haven& #39;t seen any).
Typo: messed up Kim Prather& #39;s handle in tweet #3, it is @kprather88
43/ Este hilo en Espanol; https://twitter.com/jljcolorado/status/1382838079191355394?s=19">https://twitter.com/jljcolora...
44/ OK, so it& #39;s airborne, how do we protect ourselves better?

See our scientists FAQs at http://Tinyurl.com/faqs-aerosol ">https://Tinyurl.com/faqs-aero...
45/ Reaction from higher ups in large Public Health agency (confidentially thru colleague)

They still don& #39;t think it& #39;s airborne, based on these considerations:

- there are still not enough good masks
- ventilation changes would be expensive

Not a joke, this is what I was told
46/ So far the @TheLancet piece seems to be having a high impact, thanks to everyone for bringing attention to it.

https://www.altmetric.com/details/103982946">https://www.altmetric.com/details/1...
You can follow @jljcolorado.
Tip: mention @twtextapp on a Twitter thread with the keyword “unroll” to get a link to it.

Latest Threads Unrolled:
There are a lot of misconceptions about the Turkish diaspora in Germany. Since i just finished writing an essay about this topic I thought it's time to give other people
Read more
Las mejores calculadoras online en 2020A thread http://totumat.com/2020/11/26/las-mejores-calculadoras-online-en-2020/ Cuando se estudian asignaturas que requieren de cálculos complicados, nada mejor que contar con una buena calculadora.
Read more
Mitigating #ClimateChange is as much about taking up new techs as about leaving others behindMost studies focus on #coal's global downfall but another #energy tech decline is loomingRead my new
Read more