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How can you reconstruct the lineage history of an organism without disrupting tissue organization? Introducing intMEMOIR - An optical lineage reconstruction technology developed as a collaboration with @GraceKC, @ElowitzLab, Alejandro Granados, Carlos Lois, and more. 1/
It was important for us to compare our reconstruction accuracy directly to ground-truth data. This video shows an overview of how we did this. 2/
The core technology is a “trit”, the ternary equivalent of a bit. Using integrases, such as Bxb1, individual trits can rapidly and irreversibly encode information via DNA rearrangements. These could be used to actuate differential gene expression on lineage branches. 3/
We implemented intMEMOIR in both mES cells and D. melanogaster, which we call "Drosphila memoiphila". Recording in early embryos and reading out in adult brains reveals that, only within clones, longer distance between cells correlates with greater gene expression differences. 4/
We're excited to get intMEMOIR into the hands of scientists who can use it to learn new biology. Please reach out and contact us if you think intMEMOIR can help you answer new questions. 5/5 https://www.elowitz.caltech.edu/
Paper is here: https://science.sciencemag.org/content/372/6538/eabb3099
