castiel: gay, killed after coming out
charlie bradbury: lesbian, killed violently
dean winchester: bisexual, silenced and killed
eileen leahy: deaf, discarded from the narrative
kevin tran: asian, eyes burned out and sent to hell
crowley macleod: pansexual, killed himself

rowena macleod: bisexual, sacrificed herself
adam milligan: traumatised and constantly disrespected by the narrative
michael: killed by his abusive father and being labelled as a bad guy getting what was coming
chuck shurley: bisexual, the biggest villain of the show

https://twitter.com/endversetiel/status/1331578042574131200

kaia: lesbian, killed by a spear in her side
linda tran: asian, off screen death

billie: non-binary, killed
stevie: queer, killed off for shock value
cassie robinson: love interest but never talked about again
missouri moseley, banes twins, rufus turner: killed