Morning everyone!
Here in rainy Wollongong, I’m starting my @science_a_thon #dayofscience by watering my office plants @MolHorizons @uowresearch, supervised by Dr Worm (a leaving gift from @LabSchafer)
In our lab, we are #neuroscientists, #geneticists and worm-wranglers - we use the nematode C. elegans to study how neurochemical signals tune learning & adaptive behaviour in the brain https://abs.twimg.com/emoji/v2/... draggable="false" alt="🧠" title="Gehirn" aria-label="Emoji: Gehirn">.

On this @science_a_thon #dayofscience, come on in and meet our worms! https://abs.twimg.com/emoji/v2/... draggable="false" alt="🐛" title="Käfer" aria-label="Emoji: Käfer">
Our little worms are microscopic, just 1 mm long, so we need a microscope to look at them.

Amazingly, worms have:

https://abs.twimg.com/emoji/v2/... draggable="false" alt="🧠" title="Gehirn" aria-label="Emoji: Gehirn"> 300 brain cells

https://abs.twimg.com/emoji/v2/... draggable="false" alt="🧬" title="DNA" aria-label="Emoji: DNA">~20,000 genes (not too different from humans!)

https://abs.twimg.com/emoji/v2/... draggable="false" alt="🥚" title="Ei" aria-label="Emoji: Ei"> the ability to self-fertilise i.e. make both eggs + sperm and produce clones of themselves
Phew! And now all those meetings are done, come into the lab @MolHorizons to continue my @science_a_thon #dayofscience!

We’ve got hoods, microscopes, water baths, incubators, heat blocks, pipettes... can you spot them all?? https://abs.twimg.com/emoji/v2/... draggable="false" alt="🧬" title="DNA" aria-label="Emoji: DNA">https://abs.twimg.com/emoji/v2/... draggable="false" alt="🧪" title="Test tube" aria-label="Emoji: Test tube">https://abs.twimg.com/emoji/v2/... draggable="false" alt="🔬" title="Mikroskop" aria-label="Emoji: Mikroskop">
Today @science_a_thon #dayofscience I’ll be attempting to generate some transgenic worms https://abs.twimg.com/emoji/v2/... draggable="false" alt="🐛" title="Käfer" aria-label="Emoji: Käfer"> by microinjection.

First step, making an injection mix.
Check it out https://abs.twimg.com/emoji/v2/... draggable="false" alt="👇🏽" title="Rückhand Zeigefinger nach unten (mittlerer Hautton)" aria-label="Emoji: Rückhand Zeigefinger nach unten (mittlerer Hautton)">- how much liquid https://abs.twimg.com/emoji/v2/... draggable="false" alt="💧" title="Tröpfchen" aria-label="Emoji: Tröpfchen"> do you think that is?

It’s 10 microlitres! That’s 100,000th of a litre! Whoaaaaaa
Today  @science_a_thon   #dayofscience I’ll be attempting to generate some transgenic worms https://abs.twimg.com/emoji/v2/... draggable= by microinjection. First step, making an injection mix. Check it out https://abs.twimg.com/emoji/v2/... draggable="false" alt="👇🏽" title="Rückhand Zeigefinger nach unten (mittlerer Hautton)" aria-label="Emoji: Rückhand Zeigefinger nach unten (mittlerer Hautton)">- how much liquid https://abs.twimg.com/emoji/v2/... draggable="false" alt="💧" title="Tröpfchen" aria-label="Emoji: Tröpfchen"> do you think that is? It’s 10 microlitres! That’s 100,000th of a litre! Whoaaaaaa" title="Today @science_a_thon #dayofscience I’ll be attempting to generate some transgenic worms https://abs.twimg.com/emoji/v2/... draggable="false" alt="🐛" title="Käfer" aria-label="Emoji: Käfer"> by microinjection. First step, making an injection mix. Check it out https://abs.twimg.com/emoji/v2/... draggable="false" alt="👇🏽" title="Rückhand Zeigefinger nach unten (mittlerer Hautton)" aria-label="Emoji: Rückhand Zeigefinger nach unten (mittlerer Hautton)">- how much liquid https://abs.twimg.com/emoji/v2/... draggable="false" alt="💧" title="Tröpfchen" aria-label="Emoji: Tröpfchen"> do you think that is? It’s 10 microlitres! That’s 100,000th of a litre! Whoaaaaaa" class="img-responsive" style="max-width:100%;"/>
Just headed over to @ihmri for https://abs.twimg.com/emoji/v2/... draggable="false" alt="🐛" title="Käfer" aria-label="Emoji: Käfer">microinjections! Much excite.

@science_a_thon
#dayofscience
Ok so after a short episode of dropping a small... transparent... squishy tube on the floor (twice), then searching for it (unsuccessfully) on my hands and knees...

I have finally set everything up to inject!

@science_a_thon
#dayofscience

(Photo taken prior to clumsytime)
So how does microinjection work? We fill a glass filament with a long tapered end (like a needle) with injection mix containing DNA https://abs.twimg.com/emoji/v2/... draggable="false" alt="🧬" title="DNA" aria-label="Emoji: DNA">.

Under the microscope, we move the needle veeeery carefully into the worm’s “germline”(the cells that pass on genes to progeny)... and inject!
After injections, briefly caught up with my lovely friend and colleague @AmyEMcG !

Dr Amy McGrath is an awesome crystallographer and microbiologist!

@science_a_thon
#dayofscience
Almost done with my @science_a_thon #dayofscience, now on the train home, watching the beautiful coastline in Dharawal country.

I have a pretty long (~90 min) train ride - but there’s always something to do: emails, readings or maybe even a little snoozle https://abs.twimg.com/emoji/v2/... draggable="false" alt="💤" title="Schlafsymbol" aria-label="Emoji: Schlafsymbol">
You can follow @wormychew.
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