Should guidelines be evidence-based?
The PCR test works by duplicating the genetic material in a sample to a point where a specific genetic sequence is detected.

Ct is the cycle threshold. It is the number of amplification cycles needed ...
1/n
..to see the fluorescent color which indicates the presence of the genetic sequence.

The higher the Ct it is set on, the more likely it is to detect a lower viral load. 2/n
So if a person has recovered and has a minute amount of viral debris still in his body:
by setting the Ct on 30, the viral debris won& #39;t be detected => giving a (-) test result
by setting the Ct on 40, the viral debris will be detected
=> giving a False (+) test result 3/n
The FDA& #39;s Guideline: Ct= 47 cycles
https://www.fda.gov/media/137120/download
5/n">https://www.fda.gov/media/137...
"viral culture & isolation is regarded as a gold standard or reference test against which any diagnostic index test for viruses must be measured and calibrated" 7/n
https://www.cebm.net/covid-19/infectious-positive-pcr-test-result-covid-19/">https://www.cebm.net/covid-19/...
Here& #39;s another study:
“The lowest viral load (Ct value) for which there was positive culture growth was 34.3.”
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7323671/

No">https://www.ncbi.nlm.nih.gov/pmc/artic... live viruses are found at CT values of 30 or 34 and above.

All what these tests are detecting is old debris of the virus. 9/n
Is it time to listen to the evidence and change the guidelines?

Reducing the Ct cut-off of the PCR test would reduce the false positives, the #Casedemic and the unjustified restrictions on people& #39;s lives. 10/10
On 8/n
Typo: Cut-off
Prof Carl Heneghan recommends a Ct cutoff of 30. https://twitter.com/carlheneghan/status/1303220734689501184">https://twitter.com/carlheneg...
“SARS-CoV-2 cell infectivity was only observed for RT-PCR Ct < 24 and STT < 8 days.”
STT: Symptom To Test

To diagnose:
1) PCR (+/-)
2) Ct (cycle threshold)
3)Time of symptom onset (to determine time point of infection) https://pubmed.ncbi.nlm.nih.gov/32442256/ ">https://pubmed.ncbi.nlm.nih.gov/32442256/...
Note: https://twitter.com/abirballan/status/1304621366717673472">https://twitter.com/abirballa...
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