Our #research into #pandemic multidrug-resistant E. coli O25b:H4:ST131 is FINALLY out in @FrontMicrobiol Using WGS to show companion animals represent occasional spillover hosts rather than primary reservoirs for the ST131 lineage...a thread1/7

Paper https://www.frontiersin.org/articles/10.3389/fmicb.2020.01968/full

Recently, #ST131 has emerged & spread globally. Dissemination is unclear in #Australia. There’re reports of commonality between ST131 from humans & pets. Here, using #WGS, we investigated pet-sourced E. coli on an Australia-wide level against a global collection 2/7

We used sources of isolates & compared characteristics of FQR E. coli from humans & pets, using PCR-based phylotyping & WGS. We include #genomic data from Aussie isolates over 2-years & a previous study by @genomiss in @mbiojournal https://mbio.asm.org/content/7/2/e00347-16.abstract [110 citations] 3/7

We show the epidemiological associations of these isolates is leading to the clinically relevant conclusion that the transfer of #AMR E. coli ST131 between humans & pets is much less frequent than assumed 4/7

This work showcases skills from a great collaborative team, incl.
Amanda Kidsley & Darren Trott ( @UniofAdelaide)
the Australian Group for #AMR affiliated w/ @AusAntibiotics
@beatsonlab & @MarkSchembri7
@mrsmolly01
@sam_manna3
& many more I can’t find on twitter 5/7

This project was funded by an @arc_gov_au grant w/ @Zoetis Australia as a main industry partner. This work was also supported in part by the @DeptVetAffairs. We acknowledge the assistance of the private, government & university veterinary diagnostic laboratories in Australia 6/7

Sequence read data has been submitted to the @NCBI Sequence Read Archive (SRA) under BioProject PRJNA627752

Sequence data https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA627752

de novo assemblies https://github.com/RhysWhite/ST131_19_Pets_AU
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