Where is RNA in the bacterial cytoplasm? Well, it depends on the sequence and on the species—once more, there are not general rules 😱 https://onlinelibrary.wiley.com/doi/abs/10.1002/wrna.1615
BTW one more nail in the coffin of the textbook assertion—mostly based on data on some genes of E. coli—that transcription/translation coupling & Rho-dep termination is a must for avoiding transcript chaos in the non-compartimentalized prok cytoplasm. Other bugs do differently 💪🏻
I find this concept a most inspiring part of Orna Amster-Choder’s review: Chemical properties of given RNA sequences may drive formation of well-separated subcellular domains ➡️ physical clustering of RNA is a cause, not the effect of a distinct 3D cytoplasmic architecture 👇
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