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1/5
African S. Typhimurium ST313 has 23 Lineage 2-specific pseudogenes, discovered by @DrRobKingsley in 2009, inc ybjZ.
But what is function?
In collab with Jared Honeycutt @monack_lab, we've discovered that MacB (YbjZ) influences pathogenesis of ST313
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1008763
African S. Typhimurium ST313 has 23 Lineage 2-specific pseudogenes, discovered by @DrRobKingsley in 2009, inc ybjZ.
But what is function?
In collab with Jared Honeycutt @monack_lab, we've discovered that MacB (YbjZ) influences pathogenesis of ST313
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1008763
2/5
Genomes of 285 S. Typhimurium isolates revealed lineage-specific SNPs = macA & macB are pseudogenes
Suggesting that MacAB-TolC efflux pump is functional in S. Typhimurium ST19, but defective in ST313 Lineages 1 & 2
Genomes of 285 S. Typhimurium isolates revealed lineage-specific SNPs = macA & macB are pseudogenes
Suggesting that MacAB-TolC efflux pump is functional in S. Typhimurium ST19, but defective in ST313 Lineages 1 & 2
3/5
Beautiful expts designed by Jared Honeycutt & @monack_lab show MacA variant & truncated MacB protein made ST19 sensitive to C18G antimicrobial peptide
& the ST313 macAB SNPs reduced intra-macrophage replication & fitness in murine GI tract
See @monack_lab tweet for details
Beautiful expts designed by Jared Honeycutt & @monack_lab show MacA variant & truncated MacB protein made ST19 sensitive to C18G antimicrobial peptide
& the ST313 macAB SNPs reduced intra-macrophage replication & fitness in murine GI tract
See @monack_lab tweet for details
4/5
Degradation of function of MacAB-TolC efflux pump in African @Salmonella ST313 lineages = convergent evolution
? This new adaptation linked to ability to cause systemic disease #iNTS in immunocompromised people
#PLOSPathogens @LivUni_IVES @LivUni
https://dx.plos.org/10.1371/journal.ppat.1008763
Degradation of function of MacAB-TolC efflux pump in African @Salmonella ST313 lineages = convergent evolution
? This new adaptation linked to ability to cause systemic disease #iNTS in immunocompromised people
#PLOSPathogens @LivUni_IVES @LivUni
https://dx.plos.org/10.1371/journal.ppat.1008763
5/5
We're proud of our collab with @monack_lab
Invaluable contributions from #MolecularMicrobiologists in HintonLab: Nico Wenner, @yan_salsa @implosian & @knalsal
Thanks to @wellcometrust for funding & to @MlwTrust
Graphic by @eliza_coli
#SciArt #Microbiology #scicomm
We're proud of our collab with @monack_lab
Invaluable contributions from #MolecularMicrobiologists in HintonLab: Nico Wenner, @yan_salsa @implosian & @knalsal
Thanks to @wellcometrust for funding & to @MlwTrust
Graphic by @eliza_coli
#SciArt #Microbiology #scicomm
