CEREBROSPINAL FLUID
)CSF)
من أهم العينات في المختبر و تتطلب التعامل معاها مباشرة
نلخصها في تغريدات و نسألكم عنها شويا
Specimen collection and handling
•Tube 1 – chemistries and serology
•Tube 2 – microbiology cultures
•Tube 3 – hematology

من أهم العينات في المختبر و تتطلب التعامل معاها مباشرة
نلخصها في تغريدات و نسألكم عنها شويا


•Tube 1 – chemistries and serology
•Tube 2 – microbiology cultures
•Tube 3 – hematology


•Tube 1 (chem-sero)
•Tube 2 (micro)
Tube 3 (hemo)

•Gram stain – Extremely important for early diagnosis of bacterial meningitis
•Even when well performed, 10% false negatives occur
•Use of Cytospin to concentrate specimen increases sensitivity
•Cultures- Aerobic & Anaerobic. Culture blood at same time
Organisms
Could find
•Newborns
•E. coli & group B Strep.
•Children
•Streptococcus pneumoniae
•Hemophilus influenzae
•Neisseria meningitidis
Could find
•Newborns

•E. coli & group B Strep.
•Children

•Streptococcus pneumoniae
•Hemophilus influenzae
•Neisseria meningitidis
Adults -
•Neisseria meningitidis
•Streptococcus pneumoniae
•Staph. aureus
•Candida albicans
•Neisseria meningitidis
•Streptococcus pneumoniae
•Staph. aureus
•Candida albicans
RESULT OF GRAM STAIN
Neisseria meningitidis (meningococci)
• Diplococci, lying side by side (Kidney shape)
• Intracellular, inside the neutrophils. Diplococci may occasionally be seen outside the cells and are usually few in number.

• Diplococci, lying side by side (Kidney shape)
• Intracellular, inside the neutrophils. Diplococci may occasionally be seen outside the cells and are usually few in number.

• Gram-positive
• Diplococci, lying end to end (glass shape)
•Surrounded by a capsule, which is not visible with Gram stain
• Not intracellular
• Usually many in number