1. #WBTC1 #EvoBeh2 Bat #acoustic monitoring is fantastic and has led to species discoveries worldwide. But often we are faced with trying to classify #sonograms that look like this . Can you guess what common widespread European species are recorded here? #echolocation

2. #WBTC1 #EvoBeh2 Echolocation keys point to 2 cryptic species here.

But is it that simple?

Why is it that in 20 years of extensive bat monitoring in #EbroDelta acoustic surveys suggest presence of both pipistrelles, yet Ppip has never been confirmed?

3. #WBTC1 #EvoBeh2! We used DNA high-throughput barcoding , intensive trapping, roost checks (of 400+ #batboxes and buildings), emergence surveys & passive detectors across the delta to test who was emitting the lower freq calls? a) Pppip or b) Ppyg w/ echolocation plasticity

4. #WBTC1 #EvoBeh2 There were all Ppyg! Emerging Ppyg (confirmed by ) echolocated with FME as low as 44kHz (lower than ever reported ), w/ 29% of calls in the FME range of Ppip ! 3 variables significantly affected FME plasticity: habitat, bat density, and bat rearing stage

5. #WBTC1 #EvoBeh2 Takeaway : ID classification can fail to account for echolocation plasticity. We add to a growing list of literature highlighting the behavioural and ecological extents of this #plasticity – with important implications for bat monitoring efforts. More soon