LGBT themes in animation - A thread. Feel free to suggest inclusions!
RWBY - Rooster Teeth. This popular 3D animated show boasts canonical and confirmed LGBT characters, including two of the main characters, as well as two minor characters, who are also lesbian, and a newly added transgender character!
Steven Universe - Cartoon Network. I'm sure you've heard about this gem of a show, and it's colorful cast of humanoid rock aliens. Known for the ability to tackle hard topics, this show has two lesbian leads, an asexual icon and a bisexual diamond backing it all up.
My Little Pony - Discovery Family. The show that molded the future of cartoons in the 2010's, these pastel ponies teach lessons of friendship and love, with two of the main leads ending up in a romance together, as well as two minor lesbian couples, one being a fan favorite pair.
My Little Pony: Equestria Girls.
A spin off of the former, this series of movies and specials have a canon bisexual character leading the story, as well as two other mains, and more!
Tangled The Series - Disney
The TV show that comes after the Disney movie, Tangled, we meet Cassandra, a new character in the kingdom of Corona. A fan favorite, this lovely lady was confirmed to be a lesbian!
Star vs The Forces of Evil - Disney

You know when you're having two bisexual characters leading the series, you're in for a treat. This mid-2010's animated treat gave us a bisexual princess, and her two bi love interests, as well as fan favorite Jackie.
Frozen - Disney

Many fans claim Elsa, the secondary protagonist is either a lesbian, or ace. Either is a great theory!
Adventure Time - Cartoon Network

The animated series that started it all off, this colorful drama has two bisexual main characters, who end up together in the end!
Craig Of The Creek - Cartoon Network

While not as known or popular as the others on this list, the gay themes are strong in this one. With one of the main characters showing affection for another female, that's only the tip of the iceberg.
Victor and Valentino - Cartoon Network

This show is underated, but the best part is Xoichi, a girl voiced by the lovely Cristina Vee, and the best thing is her female love interest!
She Ra - Netflix

Although I am not much of a fan of this series, I can't deny how much LGBT content is in this series.
Polly Pocket

While making this thread, I discovered the Polly Pocket animated series has bisexual leads!
The Loud House - Nick

This new Nick classic boasts two gay dads and a bisexual lead!
Miraculous Ladybug

Two pairs of supporting characters can be seen in situations that would be seen as romantic
The Hollow - Netflix

A suggestion from a follower, the MC is gay and has two gay dads.
Kipo - Netflix

This beautiful animated series marks the first time that a main character has explicitly stated they are gay.
The Casagrandes - Nick https://twitter.com/xSunnyEclipse/status/1259200484956659712?s=19
https://twitter.com/WDonut04/status/1259206819605549056?s=19
https://twitter.com/WDonut04/status/1259207028251209729?s=19
Clarence - Cartoon Network

This underrated series had a mc with two gay moms and the first on screen gay characters in a kids show.
OK KO - Cartoon Network https://twitter.com/PrincessTekki/status/1259216653105201156?s=19
Rocko's Modern Life: Static Cling

The character in focus of this special is Rachael, a MTF trans (can I say women if she's a frog?)
Twelve Forever - Netflix https://twitter.com/PrincessTekki/status/1259217699076485123?s=19
Danger and Eggs - Amazon Prime https://twitter.com/TailSweet/status/1259222092215533568?s=19
Gravity Falls - Disney

These two cops were confirmed to be in a gay relationship at the end of the series
Ducktales - Disney

One of the supporting characters has two gay dads!
DC super hero girls https://twitter.com/KalosFilms/status/1259232275172077571?s=19
Cleopatra In Space https://twitter.com/BovineBonita/status/1259233043014959104?s=19
Too Loud https://twitter.com/BovineBonita/status/1259234021650731009?s=19
https://twitter.com/JosephSaveRBUK/status/1259237206151094273?s=19
https://twitter.com/AnimaSeriesCaps/status/1259239187024355328?s=19
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