sound off on your Favorite PCR gel concentration/conditions in the comments

1.5% agarose gels at 80V for 35 minutes were absolutely My Shit in undergrad

clearly some of y'all did not deal with persistent bastard viruses that liked to throw secondary bands too faint to observe on 100V gels but just strong enough to throw off your Sanger sequencing! and i respect that

oh- and this was in 1xTAE, visualized with EtBr