TL #epilepsy is the 4th most common #neurological #disorder. #hippocampus is linked to disease severity and phenotypes like seizure frequency yet impact of the disease at the cellular level remains unclear in #humans 1/X https://twitter.com/AllenInstitute/status/1255170020990058496

We focus on the #hippocampus, a #brain area linked to the generation and spread of #seizures, and study live #brain tissue excised during temporal lobectomy in #human #epilepsy patients 2/X

We generate a number of #data modalities ( #sn_RNA-seq, sc #ephys, #morphology, spine density estimation) and look for the hallmarks of #epilepsy progression at the #SingleCell and #celltype to link to the pathophysiology and #seizures

a number of properties are affected by #epilepsy and #disease progression: #ephys #morphology and spine density. a highlight: via #sn_RNA-seq and #modeling we link disease progression in #human #hippocampus to changes in 3 ion conductances 4/X

bio-realistic #modeling of #SingleCell and #networks shows how conductance changes brought about by #epilepsy progression suffice to elicit and increase #seizure-like activity. When reverting the 3 conductances (only!), #network becomes less excitable 5/X

Congrats to @neurojoy for spearheading the study and other in my team: @ynwa6908 TomC @YinaWei Excellent example of team effort w JonathanT @KalmbachsCortex @staci_sorensen and others @AllenInstitute of exceptional importance the #Collaboration w @Gwinnr @Swedish 6/X

personal note: deeply thankful we put the pipelines @AllenInstitute toward understanding this terrible #disease a bit better. having met #epilepsy #patients and their families, they are #heroes ! thanks @AmEpilepsySoc @epilepsysociety @CureEpilepsy @epilepsyaction for your work!