Happy to finally see out our last collaboration @uosIDS ( @unisouthampton) and @IITalk with @Bioprinting_DD ( @tudresden_de) https://iopscience.iop.org/article/10.1088/1758-5090/ab8753

I am discussing a few details of the paper here below
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This work published in @Biofabrication aimed to investigate the functionality of a bioink that we developed in a previous publication https://iopscience.iop.org/article/10.1088/1758-5090/aa7e96 called 3-3-3 for its 3% concentration of Laponite-alginate-methylcellulose
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In this new paper we have looked at human bone marrow stromal cells (HBMSCs) viability after printing in 3-3-3 finding a consistent number of viable cells during 21 days of culture in the lab, with printed cells rapidly growing in number up to 7 days and then plateauing
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It was quite interesting to find that HBMSCs were functional right after printing in 3-3-3 (high alkaline phosphatase (ALP) staining in red) while mineralising over time up to 21 days in a proportional curve opposite to ALP decrease
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We looked at the possibility of 3D printed 3-3-3 material to support vascular ingrowth. We found that the synergistic effect of the loading of growth factors and endothelial cells had a major impact on blood vessels penetrating the three-dimensional porous structure
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We wanted to investigate the regenerative potential of acellular 3D printed 3-3-3 compared to some alginate controls. We found that the 3-3-3 3D printed scaffolds could self-mineralise over a period of 4 weeks, even in the absence of loaded bone-stimulating factors.
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Lastly, we 3D printed HBMSCs in 3-3-3 comparing this group with acellular and with casted bulk controls (with no pores). We found that HBMSCs printing and the implantation of the resulting 3D construct could drastically improve the formation of mineralised tissue.
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Drawbacks of the study: this platform could be only utilised for the repair of non-load-bearing sites (definitely not the femur in the leg!). Hopefully, we will be able to fix this and further implement this regenerative tool with @Tillerius and @Bioprinting_DD