Thread by @ChrisPerryMito, Now that our book chapter is submitted on reactive oxygen species regulation [...]

ChristopherPerry PhD

ChrisPerryMito

Now that our book chapter is submitted on reactive oxygen species regulation in skeletal muscle during exercise, the fun of sharing papers we loved the most can now commence! I'll start, and @cat_bellissimo can add! String to follow:

Topic 1: Xanthine oxidase as a source of ROS after exercise. Pioneering work by Ylva Hellsten from 1988 (A-V differences purine nucleotide release) up to 1997 (XO increases in endothelial cells and leucocytes post-damaging exercise

https://www.ncbi.nlm.nih.gov/pubmed/3239420
https://www.ncbi.nlm.nih.gov/pubmed/9023782

Topic 4: The incredible potential for peroxiredoxins to serve as mediators of muscle adaptation through their role as antioxidants...exciting ideas by @ajwadders in this review:

https://www.ncbi.nlm.nih.gov/pubmed/26748042

Topic 2: NADPH oxidase-derived ROS increases during contraction in single fibres, but NOT mitochondrial ROS. Several studies, but here are some favourites:

By @malcolm_mjj https://www.ncbi.nlm.nih.gov/pubmed/23050834
By @MuscleBiology @tejensen23 NOX regulates glucose uptake
https://www.ncbi.nlm.nih.gov/pubmed/31604916

Also, the 2nd study above by @MuscleBiology and @tejensen23 used a powerful in vivo model to detect NOX-2 activity

Topic 3: Emerging roles of thioredoxins as critical regulators of redox homeostasis and metabolism in muscle following exercise. It's not just about glutathione. By @KFW_Lab

https://www.ncbi.nlm.nih.gov/pubmed/?term=fisher-wellman+thioredoxin+2013

Last but not least, excellent teaching resources on the regulation of mitochondrial bioenergetics: more than just ROS and ATP production

Bioenergetics 4 by David Nicholls, Ferguson
https://www.elsevier.com/books/bioenergetics/nicholls/978-0-12-388425-1
The Bioenergetics of Exercise by P.D. Neufer https://www.ncbi.nlm.nih.gov/pubmed/28490536

Topic 6: contemporary views of oxidative stress. Let's move beyond 'ROS bad, antioxidants good'. Location, location, location. Type, source, and circuits, circuits, circuits. Examples from Dean Jones:

https://www.ncbi.nlm.nih.gov/pubmed/18684987
https://www.ncbi.nlm.nih.gov/pubmed/23861437
https://www.ncbi.nlm.nih.gov/pubmed/23946468

Topic 2 cont.: One of my all-time favourite studies on ROS-signaling feedback loops with direct protein-oxidation detection. NOX-derived ROS activates glutathione reductase to maintain glutathione: think 'ROS-antioxidant activation loop'. By
@Mito_DB
https://ncbi.nlm.nih.gov/pubmed/23341578

Topic 5: the redox-sensitive proteome ('redoxome'). Too many studies to list that reveal the specific proteins that are known to be ROS-sensitive, yet most have not been investigates int terms of how ROS regulates their activities, let alone in response to exercise. Untapped.

Topic 4b cont'd: peroxiredoxins may also serve as inducers of immune activation (role in tissue regeneration/repair?) as speculated by @ajwadders...see molecular example here of how this may happen:

https://www.ncbi.nlm.nih.gov/pubmed/?term=Riddell+JR%2C+Wang+XY%2C+Minderman+H%2C+and+Gollnick+SO.+Peroxiredoxin+1+stimulates+secretion+of+proinflammatory+cytokines+by+binding+to+TLR4.+J+Immunol+184%3A+1022-1030%2C+2010.

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