Read on Twitter
1/n
I made a list of #COVID-19 diagnostic tests that use #CRISPR-Cas technology and summarized the pre-prints! Also includes papers/articles that propose CRISPR-Cas based COVID-19 treatments. I might have missed some but here's the thread..
I made a list of #COVID-19 diagnostic tests that use #CRISPR-Cas technology and summarized the pre-prints! Also includes papers/articles that propose CRISPR-Cas based COVID-19 treatments. I might have missed some but here's the thread..
2/n
This paper utilizes specific high-sensitivity enzymatic reporter unlocking (SHERLOCK) to detect nucleic acids and a subsequent Cas12a mediated detection via fluorescence. Total test time ~57min
https://www.broadinstitute.org/files/publications/special/COVID-19%20detection%20(updated).pdf
SHERLOCK
https://www.nature.com/articles/s41596-019-0210-2
This paper utilizes specific high-sensitivity enzymatic reporter unlocking (SHERLOCK) to detect nucleic acids and a subsequent Cas12a mediated detection via fluorescence. Total test time ~57min
https://www.broadinstitute.org/files/publications/special/COVID-19%20detection%20(updated).pdf
SHERLOCK
https://www.nature.com/articles/s41596-019-0210-2
3/n
In this pre-print, using a Cas13 based strategy, they screened several functional crRNAs that can target and effectively degrade SARS-CoV-2 and H1N1 sequences in human lung epithelial cells.
https://www.biorxiv.org/content/10.1101/2020.03.13.991307v1.full.pdf
In this pre-print, using a Cas13 based strategy, they screened several functional crRNAs that can target and effectively degrade SARS-CoV-2 and H1N1 sequences in human lung epithelial cells.
https://www.biorxiv.org/content/10.1101/2020.03.13.991307v1.full.pdf
4/n
In this pre-print, they demonstrate rapid detection of #SARSCoV2 and HIV using a one-pot recipe containing all the components for nucleic acid amplification and CRISPR detection. Test time ~40min(or upto 90min)
https://www.biorxiv.org/content/10.1101/2020.03.19.998724v1.full.pdf
In this pre-print, they demonstrate rapid detection of #SARSCoV2 and HIV using a one-pot recipe containing all the components for nucleic acid amplification and CRISPR detection. Test time ~40min(or upto 90min)
https://www.biorxiv.org/content/10.1101/2020.03.19.998724v1.full.pdf
5/n
Fastest test so far. In this protocol, they use a Cas12a based strategy to perform iso-thermal amplification of nucleic acids followed by a Cas12a mediated fluorescence detection and a lateral flow readout. Testing time ~32min
https://mammoth.bio/wp-content/uploads/2020/03/Mammoth-Biosciences-A-protocol-for-rapid-detection-of-SARS-CoV-2-using-CRISPR-diagnostics-DETECTR.pdf
Fastest test so far. In this protocol, they use a Cas12a based strategy to perform iso-thermal amplification of nucleic acids followed by a Cas12a mediated fluorescence detection and a lateral flow readout. Testing time ~32min
https://mammoth.bio/wp-content/uploads/2020/03/Mammoth-Biosciences-A-protocol-for-rapid-detection-of-SARS-CoV-2-using-CRISPR-diagnostics-DETECTR.pdf
6/n
In this pre-print, they used the ssDNA cleavage activity of Cas12a to detect SARS-CoV-2 using methods similar the earlier pre-prints. The major difference was the use of saliva samples to perform the tests. Testing time ~90min
https://www.biorxiv.org/content/10.1101/2020.02.29.971127v1.full.pdf
In this pre-print, they used the ssDNA cleavage activity of Cas12a to detect SARS-CoV-2 using methods similar the earlier pre-prints. The major difference was the use of saliva samples to perform the tests. Testing time ~90min
https://www.biorxiv.org/content/10.1101/2020.02.29.971127v1.full.pdf
7/n
This article proposes the use of a CRISPR/Cas13d system that can be delivered specifically to the lungs of COVID-19 infected patients using an adeno-associated virus(AAV) to treat the infection.
https://www.nature.com/articles/s41422-020-0290-0
This article proposes the use of a CRISPR/Cas13d system that can be delivered specifically to the lungs of COVID-19 infected patients using an adeno-associated virus(AAV) to treat the infection.
https://www.nature.com/articles/s41422-020-0290-0
Also, if you want to keep up with most of the SARS-Cov-2 literature unrelated to CRISPR, check out this thread by @NakedCapsid https://twitter.com/NakedCapsid/status/1242232669657804802?s=20
